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KMID : 0545120160260122098
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Journal of Microbiology and Biotechnology
2016 Volume.26 No. 12 p.2098 ~ p.2105
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Production of Methanol from Methane by Encapsulated Methylosinus sporium
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Patel Sanjay K. S.
Jeong Jae-Hoon
Mehariya Sanjeet
Otari Sachin V.
Madan Bharat
Haw Jung-Rim
Lee Jung-Kul
Zhang Liao Yuan
Kim In-Won
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Abstract
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Massive reserves of methane (CH4) remain unexplored as a feedstock for the production of liquid fuels and chemicals, mainly because of the lack of economically suitable and sustainable strategies for selective oxidation of CH4 to methanol. The present study demonstrates the bioconversion of CH4 to methanol mediated by Type I methanotrophs, such as Methylomicrobium album and Methylomicrobium alcaliphilum. Furthermore, immobilization of a Type II methanotroph, Methylosinus sporium, was carried out using different encapsulation methods, employing sodium-alginate (Na-alginate) and silica gel. The encapsulated cells demonstrated higher stability for methanol production. The optimal pH, temperature, and agitation rate were determined to be pH 7.0, 30oC, and 175 rpm, respectively, using inoculum (1.5 mg of dry cell mass/ml) and 20% of CH4 as a feed. Under these conditions, maximum methanol production (3.43 and 3.73 mM) by the encapsulated cells was recorded. Even after six cycles of reuse, the Na-alginate and silica gel encapsulated cells retained 61.8% and 51.6% of their initial efficiency for methanol production, respectively, in comparison with the efficiency of 11.5% observed in the case of free cells. These results suggest that encapsulation of methanotrophs is a promising approach to improve the stability of methanol production.
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KEYWORD
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Methanotroph, Methylosinus sporium, Immobilization, Methane bioconversion
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